Cells exponentially growing at 25°C in unbuffered YPD medium were inoculated into fresh YPD medium buffered at pH 8 and incubated at 37°C. Extracts were prepared at the moment of the shift and 1.5 and 3 h later. At 1.5 and 3 h the p88 band was present in Phr1 …
Of the 217 homozygous mutants tested, two showed poor growth on YPD medium buffered at pH 10 (Fig. 1 and data not shown). One strain carried a mutation in MDS3, as described previously , and one carried a mutation in ORF 6.7408. Some question about Pichia (Yeast) competent cell The night before transformation, a 2‐L sterile flask containing 500 mL YPD medium was inoculated with an appropriate amount of the saturated culture and grown overnight with vigorous shaking at Remasking of Candida albicans β-Glucan in Response to (A) Growth curves of SC5314 in fresh YPD medium, 1:1 ratio fresh medium and 8-h supernatant, or with supernatant replenished, buffered at pH 4 or pH 6 at 37°C. Wild-type strain (SC5314) was grown in fresh YPD (control), 1:1 ratio fresh medium and 8-h supernatant, or with supernatant supplemented with the indicated component, buffered at pH 4 An improved secretion signal enhances the secretion of
Release from Quorum-Sensing Molecules Triggers Hyphal
Jun 01, 2020
The cells were grown in a YPD medium containing 1% yeast extract, 2% Bactopeptone, and 2% dextrose. The medium was buffered by 50 mM Mes and 50 mM MOPS, and the pH was adjusted by NaOH (5, 10). Agar plates were prepared by adding 2% agar to the YPD buffer medium at the given pH. Yeast transformation was performed as described previ-
Figures and data in Streptomyces exploration is triggered